MALDI mass spectrometry advances biologics characterization with Bruker platforms

Perfect Peptide Function is the Only Path to a Therapeutic Breakthrough. For scientists developing the next generation of peptide-based therapeutics, the challenges are clear: stability, bioavailability, and efficient intracellular delivery. At Biosynth, our peptide synthesis expertise is engineered to solve these problems. We empower your drug development with critical synthesis capabilities, including: Stability Enhancements: Expertise in incorporating D-Amino Acids, cyclization, and backbone modifications to create protease-resistant, highly stable peptides. Complex Conjugation: Seamless covalent and non-covalent linking of your peptide to virtually any cargo using advanced Click Chemistry and custom linkers. Delivery Optimization: Precision in PEGylation and lipidation to fine-tune solubility, boost cell permeability, and extend in vivo half-life. Consistent Quality: Reliable synthesis from research-grade milligrams to preclinical-grade grams, all delivered with guaranteed high purity and comprehensive analytical data. Stop compromising your novel designs due to synthesis limitations. Partner with the peptide experts who understand the science of drug delivery. Discover how our expertise can accelerate your program. https://lnkd.in/dBc_HQFV #CellPenetratingPeptides #CPPs #DrugDelivery #PeptideSynthesis #Biotechnology #DrugDiscovery #Biopharma

Peptide therapeutics offer immense potential—but their clinical success is often hindered by rapid degradation and low bioavailability. At Syngene, our scientists have developed RAFT-based diblock copolymers that shield peptides and enable controlled release, enhancing therapeutic performance. This scalable platform has already improved stability in a metabolic disorder therapy and is paving the way for next-gen peptide drug delivery systems. #SyngeneImpact: Transforming polymer chemistry into therapeutic advantage. Get in touch https://lnkd.in/gCqrUPJU #PolymerDrugDelivery #PeptideTherapeutics #RAFTpolymerization #ControlledRelease #CDMO #SyngeneInnovation #Syngene

Automated Conformational Sampling & Scoring via Hybrid Monte Carlo-Gradient Techniques for Lead Optimization This research introduces a novel accelerated workflow for molecular docking and lead optimization. By synergistically combining Hybrid Monte Carlo (HMC) sampling with gradient-based scoring functions, we achieve a 10x improvement in conformational space exploration compared to traditional methods, significantly reducing lead discovery timelines. The proposed system's ability to rapidly evaluate vast numbers of conformers and identify promising candidates holds immense practical value for pharmaceutical companies and academic researchers, with estimations indicating a potential market impact exceeding $5 billion annually. We detail a refined HMC algorithm adapted for high-throughput molecular docking, alongside an enhanced gradient-based scoring function incorporating implicit solvation effects and flexible receptor treatment. Through rigorous benchmarking against established docking protocols across diverse protein targets, we demonstrate consistent improvements in binding affinity pre https://lnkd.in/gz7Ud_95

Turning complex science into real therapeutics requires partners who can solve the toughest development challenges and supply the right materials at the right quality, every time. If you’re attending TIDES Europe, connect with Christian Stumpp, MBA, Ph.D. or Gerard Palau, PhD on LinkedIn or via the TIDES platform—we’d be glad to discuss how Biosynth can support your goals for peptide and oligonucleotide development. Our expertise in both peptide and nucleoside chemistry provides support for those developing peptide therapeutics with complex conformationally relevant peptide discovery, alongside peptide synthesis and production from R&D to GMP. Our nucleoside chemists develop and manufacture modified building blocks, phosphoramidites, and other core raw materials for oligonucleotide manufacture, and oligo drug delivery like GalNAc clusters, alongside peptide-oligo conjugation expertise for delivery. #TIDESEurope #Peptides #Oligonucleotides #Innovation #LifeScience #PeptideManufacturing

𝗘𝘅𝗽𝗹𝗼𝗿𝗲 𝗼𝗽𝘁𝗶𝗼𝗻𝘀 𝗳𝗼𝗿 𝘁𝗵𝗲 𝗮𝗰𝗰𝘂𝗿𝗮𝘁𝗲 𝗺𝗲𝗮𝘀𝘂𝗿𝗲𝗺𝗲𝗻𝘁 𝗼𝗳 𝗽𝗲𝗽𝘁𝗶𝗱𝗲 𝘁𝗵𝗲𝗿𝗮𝗽𝗲𝘂𝘁𝗶𝗰𝘀 𝗹𝗶𝗸𝗲 𝘀𝗲𝗺𝗮𝗴𝗹𝘂𝘁𝗶𝗱𝗲, 𝗶𝗻 𝘃𝗶𝘃𝗼. This technical note presents a high-sensitivity LC-MS/MS method for quantifying semaglutide in rat plasma using a triple quadrupole mass spectrometer. ⏱️ With a 10-minute workflow and a lower limit of quantitation (LLOQ) of 0.2 ng/mL, this method enables reliable detection of semaglutide at trace levels—supporting robust pharmacokinetic profiling in early drug development. 🔗: Read the full technical note, here 👉 https://sciex.li/fsyrhs #PharmaDiscovery #LCMS #Bioanalysis #DrugDevelopment #MassSpec #GLP1 #TechnoteTuesday

I’m constantly intrigued by the challenge of oral peptide delivery. The success of oral #semaglutide (#Rybelsus®) has been a landmark achievement, but the precise molecular mechanism of its absorption has remained a major question. The oral delivery of large peptides, such as semaglutide, has long been a significant hurdle in pharmaceutical sciences. While the success of oral semaglutide (Rybelsus®) is well-established, the precise molecular mechanism by which the permeation enhancer, salcaprozate sodium (SNAC), facilitates its transcellular absorption has remained partially understood. A recent publication in Nature Communications by Colston, Faivre, and Schneebeli at Purdue University provides compelling new insights into this process. Leveraging extensive constant pH molecular dynamics (CpHMD) simulations, supported by experimental evidence (NMR, DOSY, DLS), the study proposes a detailed mechanism for how semaglutide permeates the gastric epithelial barrier. Key findings suggest a dual role for SNAC: ✔️ In the aqueous phase, SNAC co-aggregates with semaglutide, which is proposed to aid in its monomerization and recruitment to the cell surface. ✔️ Within the lipid membrane, SNAC forms dynamic, fluid-like aggregates, creating localized “defects”. The study posits that semaglutide, anchored by its lipid tail, then permeates the membrane by sinking into these SNAC-filled defects. The authors describe this as a process analogous to “quicksand,” which allows the polar peptide to traverse the hydrophobic barrier without causing permanent damage to the membrane. This mechanistic insight is a significant step forward from the general concepts of pH modulation and membrane fluidization. As a medicinal chemist in the GLP-1 field, understanding these molecular-level interactions is crucial for the rational design of the next generation of oral peptide therapeutics. This work provides a plausible molecular framework that could greatly inform future efforts in developing oral formulations for other challenging peptide-based drugs. Highly recommended read for anyone in the field of peptide drug delivery and formulation science. Link to article: https://lnkd.in/e6SFbcDZ #DrugDelivery #PeptideTherapeutics #GLP1 #Semaglutide #MedicinalChemistry #Pharmaceutics #MolecularDynamics #SNAC #OralPeptides #DrugFormulation

Tackling Challenges in Peptide Therapeutics Analyzing peptides and biomolecules can be complex - but Agilent’s solutions make it simpler, precise, and reproducible: 💧 Agilent InfinityLab LC Series III Pumps provide high-precision, low pulsation flow for reproducible peptide separations with exceptional baseline stability. Combined with the Agilent Jet Weaver, which uses multilayer microfluidic technology for highly efficient mixing and minimal delay volume, this setup ensures consistently reliable peptide analyses. https://lnkd.in/gD2AcNdZ ❄️ Agilent Multisampler is well-suited for peptide analysis, offering precise, carryover-free sample handling. Its multiwash feature and refrigerated, temperature-controlled design maintain the entire autosampler at the set temperature for consistent sample stability. https://lnkd.in/gKS4A5Ys 🌡️ Agilent Multicolumn Thermostat (MCT) enhances peptide analysis by providing precise, uniform temperature control via advanced Peltier technology. Its thermal equilibration system ensures consistent column conditions, minimizing retention time drift and improving peak shape, resolution, and reproducibility—essential for accurate peptide mapping. https://lnkd.in/gTAQEakD 💡 Max-Light Flow Cell Technology offers superior light transmission and minimal optical dispersion with its fused-silica capillary design resulting in higher sensitivity and lower baseline noise. https://lnkd.in/grce9Niz 🔹 Agilent Altura Ultra Inert Columns prevent interactions with metal sites that can cause adsorption, peak tailing, or loss of sensitive peptides. This ensures sharper peaks, higher recovery, reproducible retention times, and enhanced sensitivity, even for challenging or metal-sensitive peptides. https://lnkd.in/gcqj--PW 📍 Meet us at the Peptides Therapeutics / Extractables & Leachables Summit – Hyderabad to see how Agilent can help overcome your peptide analysis challenges. #Peptides #Therapeutics #AgilentTechnologies #UHPLC #Bioanalysis

Curious about how efficient and non-invasive analytics can address stability issues in lipid nanoparticle (LNP) or biotherapeutics formulations? A publication in Nature discusses intriguing aspects of LNP stability, including characterization of aggregates using SR-DLS with the NanoFlowsizer. Check it out for valuable insights: https://lnkd.in/e4yNCv59 #LNP #non-invasive #characterization #aggregation #biotherapeutics #mRNA

“Adding the ZenoTOF 8600 system gives our scientists a faster, more selective way to solve hard problems, especially in peptide and complex matrix workflows,” noted Shane Needham, PhD, Co-Founder, President & CEO, Veloxity Labs, LLC. “We’re investing in innovation that will create an immediate impact on sponsor timelines: quicker method cycles, better tools to handle interferences and added sensitivity headroom for GLP-1 receptor agonists and other peptides. It’s a natural fit with our model of speed with purpose and our growth in capacity.” #VeloxityLabs #SCIEX #ZenoTOF8600 #Bioanalysis #PeptideTherapeutics #LCMS #AnalyticalScience #DrugDevelopment #CRO #LifeSciences https://lnkd.in/dZ5Zaji9

This is what “speed with purpose” looks like: add selectivity and sensitivity where it matters, keep documentation tight, protect timelines. Sponsors don’t want heroics at the end—they want first-pass success. #DrugDevelopment #ICHM10 #Quality